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The production method of moisture-proof mulberry silk insulation rope for live working is characterized by: using mulberry silk to thread and twist, then dyeing it orange yellow, dyeing polar dyes Pra Yellow GN and Pra Red B, with a bath ratio of 1:20-30, adjusting the pH to 4-5 with ice acetic acid, controlling the temperature and time, and after dyeing, draining and entering the waterproof finishing process. The finishing liquid is made of oil repellent and water repellent agents, such as organosilicon or fluorine-containing polymers, with a bath ratio, adjusting the pH with ice acetic acid, controlling the temperature and time, draining and drying, then finishing and shaping, and finally making the rope.